The Effect of Different Levelsof Citrus Sinensis Peel Extracton Immune System and Blood Parameters of Broilers

Nariman Miraalami, Abbas Ebrahimi, Zohreh Pourhossein, Ali Ahmad AlowQotbi

Abstract—

The experiment was conducted to evaluate the effects of different levels of Citrus Sinensis Peel Extract (CSPE) onthe blood parameters of broilers. Four hundred Ross 308 strain day old broiler in a completely randomized design with five treatments (fourreplicates per treatmentand each replicate had 20 chicks) were categorized. Each treatment used either regulatory dietincluding 1000 ppm and 1250 ppm CSPE in the drinkingwater and in two periodsof 1^st to 21^stday and 1^st to 42^ndday and base diet without any additive for six weeks. Data analysis was performed using SAS software andmean comparison was conducted by Duncanmethod. The mean titer of Newcastle Disease (ND) in days 7 and 14 wasnot significantly different (p>0.05). While themean of NDtiter on days 28, 35and 42was significantly different (p<0.05). Themean ofAvian Influenza (AI) titer ondays 4, 14, 28 and 42 wassignificantly different (p<0.05). The results determined thatusing different levels of CSPE hassignificant effects on blood parameters (P<0.05) but the triglyceride concentration in the experimentaltreatment was not significantlydifferent (P>0.05). The results determined thatcholesterol, Low Density Lipoprotein (LDL), High DensityLipoprotein (HDL), Low Density Lipoprotein (VLDL) and LDL/HDL atthe rearing period was significantlyinfluenced by experimental treatments(P<0.05).

Keywords— Blood parameters, Broilers, Citrus Sinensis Peel Extract, Immune System.